The following are Golgi stains from a mouse brain. The brain was stained and then cut using a freezing microtome. The coronal sections are from the mouse's cerebellum. The sections are relatively thick cuts, about 100-150µm. To view the images, simply click the thumbnails. |
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A size comparison view of the coronal section stains | |
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A full view of the Golgi stained cerebellum. | |
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A view of some nice purkinje cell bodies and axons. Viewed at 20x . Cover slipped with SafeClear and no amber. | |
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A view of a collection of purkinje cell axons, without the bodies, demonstrating the magic of the Golgi stain. Viewed at 20x . Cover slipped with SafeClear and no amber. | |
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A collection of purkinje cell bodies and axons viewed at 20x. Notice the Silver Nitrate stained every axon of the cells making it difficult to view the axons individually. Yet another anomaly of the Golgi stain chemistry. Viewed at 20x. Treated in SafeClear and cover slipped with amber. |
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A single stained purkinje cell body and axon. This appears to be a view from below the axons, looking up at the cell body. Viewed at 40x. Treated in SafeClear and cover slipped with amber. |
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A slightly better representation of the purkinje cell bodies and axons. Notice the difference in staining of the axons compared to the previous photos. The coloring difference is attributed to a slightly thinner section slice. Viewed at 20x. Treated in SafeClear and cover slipped with amber. | |